Cyclic Citrullinated Peptide Ab High Purity Specifications for Lab Sourcing and Manufacturing Certification

Core Molecular Specs & Technical Index

Cyclic citrullinated peptide antibody (anti-CCP) is a high-purity synthetic peptide reagent designed for diagnostic assay development, autoimmune research, and cosmetic ingredient validation. This product targets B2B buyers including peptide manufacturers, contract research organizations (CROs), cosmetic formulation labs, and bulk raw material wholesalers. The core value of cyclic citrullin peptide ab lies in its exceptional specificity for citrullinated epitopes, enabling precise detection of anti-CCP antibodies in rheumatoid arthritis diagnostics and quality control of citrullinated peptide-based cosmetic actives.

Our cyclic citrullin peptide ab is synthesized via solid-phase peptide synthesis (SPPS) with Fmoc chemistry, achieving a minimum purity of 98% by HPLC. The peptide is lyophilized as a white powder with a molecular weight range of 1,500–3,500 Da, depending on the specific citrullinated sequence. Solubility is optimized in deionized water or phosphate-buffered saline (PBS) at pH 7.4, with a recommended storage condition of -20°C for long-term stability (≥24 months). Key technical indices include:

• Purity: ≥98% by reverse-phase HPLC with UV detection at 214 nm.
• Peptide Content: 70–85% by amino acid analysis (AAA).
• Endotoxin Level: <0.1 EU/mg for cell-based assays.
• Solubility: ≥10 mg/mL in PBS or water.
• Batch-to-Batch Consistency: CV <5% for molecular weight and retention time.

Industry data from the Peptide Therapeutics Foundation (2023) indicates that high-purity cyclic citrullinated peptides (≥98%) reduce false-positive rates in diagnostic ELISA kits by 40% compared to standard-grade reagents, directly impacting clinical accuracy and regulatory compliance.

Manufacturing & Quality Control

The production of cyclic citrullin peptide ab follows a rigorous GMP-compliant process to ensure batch reproducibility and endotoxin-free output. Synthesis begins with automated SPPS on a Rink amide resin, incorporating citrulline residues at specific positions. After cleavage and deprotection, the crude peptide undergoes cyclization via disulfide bond formation between cysteine residues, followed by preparative HPLC purification. Quality control includes:

• HPLC Purity Analysis: Single peak with retention time within ±0.2 minutes of reference standard.
• Mass Spectrometry: ESI-MS or MALDI-TOF confirming exact molecular weight (±1 Da).
• Amino Acid Composition: AAA verification of citrulline content.
• Residual Solvent Test: GC-MS for TFA, acetonitrile, and DMF below ICH limits.
• Stability Study: Accelerated stability at 40°C/75% RH for 4 weeks.

Certifications include ISO 9001:2015 for quality management, GMP compliance for diagnostic raw materials, and a Certificate of Analysis (CoA) with each batch. Third-party testing by an accredited lab (e.g., SGS or Eurofins) is available upon request for bulk orders.

Commercial Application Scenarios

Cyclic citrullin peptide ab serves three primary B2B applications:

Cosmetic Formulation: Used as a positive control in anti-aging ingredient testing. Cosmetic labs incorporate cyclic citrullin peptide ab into ELISA-based assays to verify the citrullination-inhibiting activity of peptides like acetyl hexapeptide-8. This ensures that anti-wrinkle actives do not trigger unintended immune responses, supporting safe product claims for regulatory submissions.

Lab Research: Academic and CRO labs use cyclic citrullin peptide ab for rheumatoid arthritis biomarker studies. It enables quantification of anti-CCP antibodies in patient sera, with high specificity (98%) for early diagnosis. Bulk orders of 10–100 mg are common for multi-assay validation.

Bulk Wholesale: Peptide distributors source cyclic citrullin peptide ab in kilogram quantities for kit manufacturing. The product is supplied as lyophilized powder in sealed vials, with custom packaging options (e.g., 1 g, 5 g, 10 g) and competitive pricing for volumes above 100 g.

cyclic citrullin peptide ab VS Ordinary Low-Grade Peptides

Bulk Purchase Selection Guide

When sourcing cyclic citrullin peptide ab for lab or manufacturing, avoid common pitfalls such as relying on low-purity batches that cause assay variability or selecting suppliers without third-party testing. Key selection standards include:

• Verify Purity Data: Request HPLC chromatograms and mass spec reports for each batch.
• Check Endotoxin Levels: For cell-based work, ensure <0.1 EU/mg.
• Assess Solubility: Test in your buffer system; some peptides require DMSO pre-dissolution.
• Review Storage Conditions: Confirm lyophilized powder stability at -20°C.
• Ask for References: Request case studies from cosmetic or diagnostic clients.

A buyer checklist includes: CoA with batch number, MSDS, stability data, and a sample for in-house validation. For bulk orders (≥100 g), negotiate a discount of 15–20% and request free shipping for international orders.

Core Product Advantages

Our cyclic citrullin peptide ab offers four key benefits for B2B buyers:

Purity: ≥98% ensures minimal cross-reactivity in ELISA and Western blot assays, reducing false-positive rates by up to 40% compared to standard-grade peptides. This is critical for diagnostic kit manufacturers requiring FDA or CE marking.

Stability: Lyophilized powder maintains integrity for 24+ months at -20°C, with accelerated stability data supporting long-term storage. This reduces inventory risk for wholesalers.

Cost Performance: Bulk pricing at $50–$80 per gram (for 100 g orders) offers a 30% savings over competitors, with no compromise on purity. Custom sequences (e.g., cyclic citrullinated peptides with specific modifications) are available at a 10% premium.

Technical Support: Our team provides free consultation on assay optimization, buffer compatibility, and regulatory documentation. This includes a dedicated account manager for bulk clients and a 24-hour response time for technical queries.

Frequently Asked Questions

Q1: What is the recommended storage condition for cyclic citrullin peptide ab after reconstitution?
After reconstitution in PBS or water, the peptide should be stored at -20°C in aliquots to avoid freeze-thaw cycles. For short-term use (within 1 week), storage at 4°C is acceptable. Avoid repeated freezing and thawing, as this can degrade the peptide and reduce assay sensitivity.

Q2: Can cyclic citrullin peptide ab be used in cosmetic formulation testing?
Yes, it is commonly used as a positive control in ELISA-based assays to evaluate the anti-citrullination activity of cosmetic peptides. However, it is not intended for direct use in cosmetic products applied to skin. For formulation testing, ensure the peptide is diluted to a non-toxic concentration (e.g., 1–10 µg/mL) in a compatible buffer.

Q3: How do I verify the batch-to-batch consistency of cyclic citrullin peptide ab?
Request the Certificate of Analysis (CoA) for each batch, which includes HPLC purity, mass spectrometry data, and amino acid analysis. Additionally, perform an in-house ELISA using a standard anti-CCP antibody to compare signal intensity across batches. A CV of less than 5% in absorbance values indicates acceptable consistency.